SARF015: The aetiology and epidemiology of PD, HSMI and CMS in Scotland

Start Date: 1/11/05
End Date:  31/10/07
Main Contractor(s): Institute of Aquaculture, University of Stirling
Other Sponsor(s): SQS, Shetland Aquaculture, University of Stirling, FRS Marine Laboratory, Aquatic Diagnostic Ltd.

Objectives

1. Is CMS infectious? A project is already under way to try to transmit CMS in Atlantic salmon, in order to determine if the disease is infectious. Samples from these fish will be made available to this proposed project.

2. Production of monoclonal antibodies to PD virus and optimisation of tests to detect antigen and anti-PD antibody in Atlantic salmon.

2.1 Production of anti-PD virus antibodies

2.2 Optimisation of IHC to detect PD virus

2.3 Optimisation of IFAT to detect PD virus

2.4 Optimisation of ELISAs to detect PD antibodies and PD antigen in salmon

2.5 Development of Lateral Flow kits to detect PD virus

2.6 Development of Lateral Flow kits to detect PD antibodies in fish

2.7 Development of a Bioplex assay to detect and quantify PD virus in serum

3. Determine the prevalence of PD in Scotland using the tests developed in 2.

4.Determine if the PD virus antigen is always, sometimes or never present in CMS and HSMI affected fish

5. Identification of cell types involved in the tissue responses to CMS, HSMI and PD.

Summary

This proposal will extend the current state of knowledge on three significant heart diseases in fish in Scotland so that differential diagnosis can be achieved and the prevalance of pancreas disease (PD) infection within Scottish fish farms established. Confirmation that cardiomyopathy syndrome (CMS) is infectious, or not, will be addressed. Finally the identity of the cell types involved in CMS, heart and skeletal muscle inflammation (HSMI) and PD will be established providing much needed basline information about the category of disease. This proposal is very relevant to the recent SARF call ' Aetioloy and epidemiology of PD and similar pathologies HSMI and CMS' and the results will be disseminated widely to assist in the diagnosis and control of these significant emerging diseases.